Technical Data
C0075-13B
CA19-9 Cancer Antigen (Pancreatic, GI or Ovarian Tumor Antigen, Sialyl Lewis a)
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Technical Data |
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C0075-13B |
CA19-9 Cancer Antigen (Pancreatic, GI or Ovarian Tumor Antigen, Sialyl Lewis a) |
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Description: CA19-9 is a carbohydrate antigenic determinant identified as a sialylated lacto-N-fucopentose II, an oligosaccharide biochemically related to the Lewis A blood group antigen. In malignant tissues positive staining of tumor cells is observed in most adenocarcinomas of the pancreas and stomach and in a large proportion of colon and gall bladder tumors. The antigen is also expressed in approximately half of primary and metastatic ovarian tumors. In normal tissues positive staining is also observed in columnor epithelium of the pancreas stomach, liver, gall bladder and in bronchial glands of the lung. Applications: Suitable for use in Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunohistochemistry (Frozen and parrafin): 1:10-1:40 Note: A minimum incubation time of 1 hour is recommended. Does not require protein digestion pre-treatment of paraffin sections. Does not require antigen retrieval using heat treatment prior to staining of paraffin sections. Optimal working dilutions to be determined by researcher. Recommended Secondary Reagents: I1904-65W: IgG, F(ab’)2 (HRP) Pab RbxMo I1904-66C: IgG, F(ab’)2, X-Adsorbed (HRP) Pab RbxMo Recommended Positive Control Tissue: Adenocarcinoma of colon Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
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