Technical Data
C0104-70C
CACNA2D1 (Voltage-dependent Calcium Channel Subunit alpha-2/delta-1, Voltage-gated Calcium Channel Subunit alpha-2/delta-1, Voltage-dependent Calcium Channel Subunit alpha-2-1, Voltage-dependent Calcium Channel Subunit delta-1, MHS3, CCHL2A, CACNL2A)
Description:
CACNA2D1 is a member of the alpha-2/delta subunit family, a protein in the voltage-dependent calcium channel complex. Calcium channels mediate the influx of calcium ions into the cell upon membrane polarization and consist of a complex of alpha-1, alpha-2/delta, beta, and gamma subunits in a 1:1:1:1 ratio. Research on a highly similar protein in rabbit suggests the protein described in this record is cleaved into alpha-2 and delta subunits.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:16,000
Western Blot: 0.03-0.1ug/ml, observed in human brain (cerebellum) lysates on ~140kD bands
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanGoat
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to C-QEIFNKYNKDKKVR, from CACNA2D1, at the internal region of the protein (NP_000713.2).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Specificity:
Recognizes human CACNA2D1. Species Crossreactivity: Mouse. Species sequence homology: Bovine, canine, porcine and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Neely GG et.al. A genome-wide Drosophila screen for heat nociception identifies a2d3 as ann evolutionarily conserved pain gene. Cell. 2010 Nov 12;143(4):628-38.