Technical Data
C0107-02
Cadherin 14, 15, M
Description:
M-cadherin belongs to the cadherin family of proteins, which are calcium-dependent, protease-sensitive molecules that mediate homotypic cell-cell adhesion. M-cadherin is highly expressed in developing and regenerating skeletal muscles.1 In postnatal mature myofibers and after completion of myotube formation in vitro, M-cadherin expression is downregulated, but the protein remains present in quiescent satellite cells.2 M-cadherin is also present in the contactus adherens in the granular cell layer of the cerebellar glomerulus.3 Studies of skeletal muscle and cerebellum in M-cadherin-null mutant mice have demonstrated that other cadherins such as N-cadherin and R-cadherin may compensate for absent M-cadherin function.4 Although the calculated molecular weight of M-cadherin is ~86kDa, the apparent molecular molecular weight is ~130kDa due to glycosylation.

Applications:
Suitable for use in ELISA, Western Blotting. Other applications not tested.

Recommended Dilution:
ELISA: 0.1–1ug/mL
Western Blotting: 1–3ug/mL
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceMouseRabbit
Concentration:
0.25mg/ml
Immunogen:
Synthetic peptide derived from the C terminal region of the mouse M-Cadherin (Cadherin 14, Cadherin 15) protein.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.
Specificity:
Speci c for the mouse M-Cadherin protein. On Western blots, it identi es a single band at ~130kD. Reactivity has been con rmed with fetal mouse muscle homogenates.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Donalies M, et al. Proc Natl Acad Sci USA 88(18): 8024-8028, 1991.
2. Beauchamp JR, et al. J Cell Biol 151(6): 1221-1234, 2000.
3. Bahjaoui-Bouhaddi M, et al. J Comp Neurol 378(2): 180-195, 1997.
4. Hollnagel A, et al. Mol Cell Biol 22(13): 4760-4770, 2002.