Technical Data
Cadherin 6 (K-Cadherin, Fetal Kidney, CDH6)
Cadherins constitute a family of transmembrane glycoproteins involved in Ca2+-dependent cell-cell interactions. The members of this family are differentially expressed in various tissues. They function in the maintenance of tissue integrity and morphogenesis. The cadherins generally contain five extracellular repeats, a transmembrane domain and a cytoplasmic tail that binds to the catenin family of cytoskeletal anchoring proteins which also function as signal transducers. The extracellular domains are responsible for the specificity of homophilic interactions between cells expressing the same cadherin. Cadherins are divided into type I and type II subgroups. Type I cadherins include epithelial cadherin (E-cadherin, cadherin-1 or uvomorulin), neural cadherin (N-cadherin or cadherin-2), placental cadherin (P-cadherin or cadherin-3) and retinal cadherin (R-cadherin or cadherin-4). Kidney cadherin (K-cadherin or cadherin-6) and osteoblast cadherin (OB-cadherin or cadherin-11) are type II cadherins. Cadherin 6 is expressed in the proximal tubule epithelium of the kidney from which most renal cell carcinomas are derived.

Suitable for use in Immunohistochemistry, Western Blot and Immunocytochemistry Other applications not tested.

Recommended Dilutions:
Immunohistochemistry (Frozen): 1:25-1:50 using a PBS buffer with 0.1mM CaCl2, 0.1mM MgCl2 and avidin-biotinylated horseradish peroxidase complex as the detection reagent.
Western Blot: 1:25-1:250
Optimal dilutions to be determined by researcher.

Sp2/0 myeloma cells with spleen cells from Balb/c mice.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
100ug-20CBlue IceHumanMouse
Human Cadherin 6, extracellular domain, GST fusion protein
Supplied as a liquid in PBS, 0.09% sodium azide.
Recognizes the extracellular domain of human Cadherin 6. Species Crossreactivity: rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Shimazui, T., et al., Clin. Cancer Res. 4: 2419-2424 (1998). 2. Shimazui, T., et al., Eur. Urol. 38: 331-338 (2000).