		Technical Data
C0107-05A	Cadherin 6 (K-Cadherin, Fetal Kidney, CDH6)

Description:
The cadherin gene family encodes calcium-dependent adhesion molecules that promote homophilic interactions among cells. All cadherins are single-pass transmembrane proteins with a variable number of extracellular domains (EC; 110 amino acids long). Classical cadherins consist of five EC domains and a conserved cytoplasmic domain and are divided in type I and type II subgroups. Type I comprises cadherin E, N, P, and R. Type II include cadherin-5, -6, -8, -11, and -12. During embryogenesis differential expression of cadherins can drive morphogenesis by stimulating cell aggregation, defining boundaries between groups of cells and promoting cell migration. Cadherin-6 seems to be required for the early aggregation of induced mesenchymal cells and their subsequent conversion to epithlium.

Applications:
Suitable for use in Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry (Frozen): Paraformaldehyde fixed, with 0.2% Triton X-100 in PBS. Incubate for 1 hr at RT or overnight at 4°C.
Optimal dilutions to be determined by the researcher.

Positive Control:
Embryonic kidney (e.g. establishment of developmental pattern of nephrons).

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgG1	5F95	Supernatant

Size		Storage	Shipping	Source	Host
1ml		-20°C	Blue Ice		Mouse

Concentration:

Not Determined

Immunogen:

GST fusion protein corresponding to part of the extracellular domain of Cadherin 6

Purity:

Supernatant

Form

Supplied as a liquid, 0.09% sodium azide.

Specificity:

Recognizes Cadherin 6. Species Crossreactivity: human

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Schalken JA et al.: Expression of cadherin-6 as a novel diagnostic prognosis of patients with E-cadherin-absent renal cell carcinoma. Clin. Cancer Res. 4, 2419-2424 (1998). 2. Schalken JA et al.: Complex cadherin expression in renal cell carcinoma. Cancer Res. 56, 3224-3237 (1996)