Technical Data
Cadherin 6 (K-Cadherin, Fetal Kidney, CDH6)
The cadherin gene family encodes calcium-dependent adhesion molecules that promote homophilic interactions among cells. All cadherins are single-pass transmembrane proteins with a variable number of extracellular domains (EC; 110 amino acids long). Classical cadherins consist of five EC domains and a conserved cytoplasmic domain and are divided in type I and type II subgroups. Type I comprises cadherin E, N, P, and R. Type II include cadherin-5, -6, -8, -11, and -12. During embryogenesis differential expression of cadherins can drive morphogenesis by stimulating cell aggregation, defining boundaries between groups of cells and promoting cell migration. Cadherin-6 seems to be required for the early aggregation of induced mesenchymal cells and their subsequent conversion to epithlium.

Suitable for use in Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry (Frozen): Paraformaldehyde fixed, with 0.2% Triton X-100 in PBS. Incubate for 1 hr at RT or overnight at 4C.
Optimal dilutions to be determined by the researcher.

Positive Control:
Embryonic kidney (e.g. establishment of developmental pattern of nephrons).

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
1ml-20CBlue IceMouse
Not Determined
GST fusion protein corresponding to part of the extracellular domain of Cadherin 6
Supplied as a liquid, 0.09% sodium azide.
Recognizes Cadherin 6. Species Crossreactivity: human
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Schalken JA et al.: Expression of cadherin-6 as a novel diagnostic prognosis of patients with E-cadherin-absent renal cell carcinoma. Clin. Cancer Res. 4, 2419-2424 (1998). 2. Schalken JA et al.: Complex cadherin expression in renal cell carcinoma. Cancer Res. 56, 3224-3237 (1996)