Technical Data
Cadherin 6 (K-Cadherin, Fetal Kidney, CDH6)
The cadherin gene family encodes calcium-dependent adhesion molecules that promote homophilic interactions among cells. All cadherins are single-pass transmembrane proteins with a variable number of extracellular domains (EC; 110aa long). Classical cadherins consist of five EC domains and a conserved cytoplasmic domain and are divided in type I and type II subgroups. Type I comprises cadherin E, N, P and R. Type II include cadherin-5, -6, -8, -11 and -12. During embryogenesis differential expression of cadherins can drive morphogenesis by stimulating cell aggregation, defining boundaries between groups of cells and promoting cell migration. Cadherin-6 seems to be required for the early aggregation of induced mesenchymal cells and their subsequent conversion to epithlium.

Suitable for use in Western Blot, Immunocytochemistry, Flow Cytometry and ELISA. Other applications not tested.

Recommended Dilutions:
Western Blot: 0.1-0.2ug/ml. The detection limit is ~10ng/lane under non-reducing and reducing conditions.
Immunocytochemistry: 10ug/ml detects human Cadherin 6 in MG63 human osteosarcoma cells. Cells were fixed with PBS, 4% paraformaldehyde for 20 minutes at RT and blocked with PBS, 10% normal donkey serum, 0.1% Triton X-100, 1% BSA for 45 minutes at RT. After blocking, cells were incubated with diluted C0107-05B for 3 hours at RT followed by donkey anti-sheep lgG at RT for 1 hour. Between each step, cells were washed with PBS, 0.1% BSA.
Flow Cytometry: 50ug/ml. 10ul labels 1-2.5x10e5 cells in a total reaction volume 200ul. Tested on MG63 human osteosarcoma cells.
Direct ELISA: 0.5-1.0ug/ml. The detection limit is ~1ng/well.
Optimal dilution determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months after receipt at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabIgGAffinity Purified
100ug-20CBlue IceHumanSheep
~0.2mg/ml (after reconstitution)
Rcombinant protein corresponding tohuman Cadherin 6, extracellular domain expressed in NS0 cells
Purified by immunoaffinity chromatography.
Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Recognizes human Cadherin 6. In direct ELISAs and Western Blot, shows < 5% crossreactivity with recombinant human Cadherin 8, Cadherin 11 and Cadherin 12.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.