Technical Data
C0107-05B
Cadherin 6 (K-Cadherin, Fetal Kidney, CDH6)
Description:
The cadherin gene family encodes calcium-dependent adhesion molecules that promote homophilic interactions among cells. All cadherins are single-pass transmembrane proteins with a variable number of extracellular domains (EC; 110aa long). Classical cadherins consist of five EC domains and a conserved cytoplasmic domain and are divided in type I and type II subgroups. Type I comprises cadherin E, N, P and R. Type II include cadherin-5, -6, -8, -11 and -12. During embryogenesis differential expression of cadherins can drive morphogenesis by stimulating cell aggregation, defining boundaries between groups of cells and promoting cell migration. Cadherin-6 seems to be required for the early aggregation of induced mesenchymal cells and their subsequent conversion to epithlium.

Applications:
Suitable for use in Western Blot, Immunocytochemistry, Flow Cytometry and ELISA. Other applications not tested.

Recommended Dilution:
Western Blot: 0.1-0.2ug/ml. The detection limit is ~10ng/lane under non-reducing and reducing conditions.
Immunocytochemistry: 10ug/ml detects human Cadherin 6 in MG63 human osteosarcoma cells. Cells were fixed with PBS, 4% paraformaldehyde for 20 minutes at RT and blocked with PBS, 10% normal donkey serum, 0.1% Triton X-100, 1% BSA for 45 minutes at RT. After blocking, cells were incubated with diluted C0107-05B for 3 hours at RT followed by donkey anti-sheep lgG at RT for 1 hour. Between each step, cells were washed with PBS, 0.1% BSA.
Flow Cytometry: 50ug/ml. 10ul labels 1-2.5x10e5 cells in a total reaction volume 200ul. Tested on MG63 human osteosarcoma cells.
Direct ELISA: 0.5-1.0ug/ml. The detection limit is ~1ng/well.
Optimal dilution determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanSheep
Concentration:
~0.2mg/ml
Immunogen:
NS0-derived recombinant human Cadherin 6, extracellur domain.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Specificity:
Recognizes human Cadherin 6. In direct ELISAs and Western Blot, shows < 5% crossreactivity with recombinant human Cadherin 8, Cadherin 11 and Cadherin 12.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.