Technical Data
C0107-07
Cadherin 11 (OB-Cadherin, Osteoblast, CDH11)
Description:
Cadherins are a large family of Ca(2+)-dependent cell-cell adhesion molecules. Classical cadherins contain ve EC domains and a conserved cytoplasmic domain. Based on amino acid alignment, classical cadherins are divided into type I and II subgroups. Type I cadherins, including cadherins E (epithelial), N (neural), P (placental), and R (retinal) differ from type I cadherins in their speci c amino acid sequences. Type II cadherins include human cadherin-5, -6, -8, -11, and -12. Cadherin 11 (OB-Cadherin) is a marker of the loosely connected and migratory cellular elements of the mesenchyme and a strong expression has been noted in brain, spinal cord, bone marrow and bone cells, and is regulated in human endometrial glandular epithelial and stromal cells. A variant Cadherin 11 gene (variant form) encodes a truncated protein with an unusual cytoplasmic domain not present in other cadherins. The variant protein is generated by alternative splicing, utilizing a 179 bp intronic sequence. Recently, Cadherin 11 mRNA and protein were found to be expressed in most invasive breast cancer cell lines tested, but not in non-invasive cell lines, suggesting that cadherin-11 may be a marker for aggressive, invasive tumor subsets.

Applications:
Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Western Blot: 2-4ug/ml
Immunoprecipitation: 5ug. Native, human; Not tested for mouse.
Immunohistochemistry (Frozen): 5-10ug/ml. Incubate for 30-60 minutes at RT or overnight at 4C.
Optimal dilutions to be determined by the researcher.

Positive Control:
MDA.MB231 cells (human) and mouse L broblast lysates.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG1,k3G41Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
~0.5mg/ml
Immunogen:
Recombinant protein corresponding to an intracellular domain of human Cadherin 11.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
Specificity:
Recognizes the ~110kD human Cadherin 11 protein. Crossreactivity with other cadherin family members has not been observed. Species Crossreactivity: mouse
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Okazaki, M., et al., J. Biol. Chem. 269(16): 12,092-12,098 (1994). 2. Pishvaian, M.J., et al., Cancer Res. 59(4): 947-952 (1999).