		Technical Data
C0107-09	Cadherin 11 (OB-Cadherin, Osteoblast, CDH11)

Description:
Cadherin-11, a mesenchymal adherin,2 is localized to the cell membrane in a detergent-soluble complex, where it associates with a-and b-catenin , and may facilitate tumor cell invasion and metastasis.4 Cadherin-11 exists as two alternatively spliced forms, cadherin-11 (OB cadherin) and cadherin-11 variant (OB-cadherin-2). The variant form is a truncated protein with an unusual cytoplasmic domain, that is not present in other cadherins.3 Both wildtype and variant form of cadherin-11 are expressed in invasive and poorly differentiated breast cancer cell lines.4 A study has shown that the presence of cadherin-11 splice variant promotes the invasion of cadherin-11 positive breast cancer cells.1

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 0.1-1ug/ml
Western Blot: 1-3ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
storglyMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		4°C (-20°C Glycerol)	Blue Ice	Human	Rabbit

Concentration:

0.25 mg/ml

Immunogen:

Synthetic peptide derived from the C-terminal region of the human Cadherin-11 Variant (OB-cadherin-2) protein.

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a liquid in PBS, 0.1% sodium azide.

Specificity:

Recognizes the C terminal region of human Cadherin-11 Variant protein. On Western blots, it identi es a single band at ~85kD. Western blots using wildtype cadherin-11 transfected HEK293 cells yielded negative results. Reactivity has been con rmed with Cadherin-11 Variant transfected human HEK293 cell lysates.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Feltes CM, et al. Cancer Res 62(22): 6688-6697, 2002. 2. Hoffmann I, et al. Dev Biol 169(1): 337-346, 1995. 3. Okazaki M, et al. J Biol Chem 269(16): 12092-12098, 1994. 4. Pishvaian MJ et al. Cancer Res 59(4): 947-952, 1999.