Technical Data
C0107-10C
Cadherin, pan
Description:
Cadherins are calcium dependent cell adhesion molecules, which play an important role in the growth and development of cells via the mechanisms of control of tissue architecture and the maintenance of tissue integrity. In adhesion junctions, cadherins are bound to beta and gamma catenins, which in turn bind to alpha catenin, an actin binding protein. Cadherins play important role in tumor invasion and metastasis.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 2-4ug/ml for 2 hours at RT.
Immunohistochemistry (Formalin-fixed, paraffin-embedded): 1:100 for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
Optimal dilutions to be determined by the researcher.

Positive Control:
MCF-7 cells, tonsil or squamous epithelium.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
250ul4C (-20C Glycerol)Blue IceChickenRabbit
Concentration:
~0.1mg/ml
Immunogen:
Synthetic peptide from the C-terminus of chicken N-Cadherin. Cellular Localization: Cell membrane.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.6, 0.2% BSA, 15mM sodium azide, before the addition of glycerol to 40%.
Specificity:
Recognizes the C-terminal of chicken Cadherin, pan at ~120kD. Species Crossreactivity: Human, mouse, rat, canine, monkey, bovine and Xenopus.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.