Technical Data
C0108-01C
Cadherin 5 (L-CAM, Epithelial Cell Adhesion Molecule, Vascular Endothelial, VE, CDH5, CD144) (FITC)
Description:
Human vascular endothelium cadherin is an endothelium-specific cadherin localized at the intercellular junctions. Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. This cadherin may play an important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. It associates with alpha catenin forming a link to the cytoskeleton.

Applications:
Suitable for use in Flow Cytometry, Western Blot, Immunohistochemistry, and Immunoassays. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 1:10
Western Blot: 1:10
Immunohistochemistry (frozen): 1:10
Optimal dilutions to be determined by the researcher.

Storage and Stability:
For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light.
TypeIsotypeCloneGrade
MabIgG2a9G137Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
As reported
Immunogen:
Human Cadherin 5.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, 1% BSA, 0.02% sodium azide. Labeled with fluorescein isothiocyanate (FITC).
Specificity:
Recognizes human VE-cadherin.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Navarro, P et al; Catenin-dependent and -independent functions of vascular endothelial cadherin. J Biol Chem 1995, 270: 30965 2. Lampugnani, MG et al; The molecular organization of endothelial cell to cell junctions: differential association of plakoglobin, beta-catenin and alpha-catenin with vascular endothelial cadherin (VE-cadherin). J Cell Biol 1995, 129: 203 3. Martin-Padura, I et al; Expression of VE (vascular endothelial)-cadherin and other endothelial-specific markers in haomangiomas. J path 1995, 175: 51 4. Breviario, F et al; Functional properties of human vascular endothelial cadherin (7B4/Cadherin-5), an endothelium-specific cadherin. Arterioscler Thromb 1995, 15: 1229