Technical Data
C0108-25D
Cadherin E (Cadherin 1, E-Cadherin, L-CAM, Epithelial Cell Adhesion Molecule, Uvomorulin, CDH1)
Description:
Several major families of cell adhesion molecules can be distinguished. These include the cadherins and N-CAMs, which play a crucial role in cell recognition and adhesion, while integrins are implicated in the adherence of cells to the extracellular matrix.

Cadherins constitute a family of proteins, the members of which are differentially expressed in the different tissues. One of the best characterized members is E-cadherin, which is prevalent in epithelial tissues. It has been shown to play a crucial role in the process of tumor cell metastasis. NCAM’s (neural cell adhesion molecules) exist in at least three forms of plasma membrane plycoproteins, which are expressed in a variety of tissues including most nerve cells.

The integrins, finally, form a large family of glycosylated transmembrane proteins that act as dimers of an a- and a beta-subunit in interconnecting the cytoskeleton and the extracellular matrix.

Applications:
Suitable for use in Immunohistochemistry (frozen sections), Immunocytochemistry, and Western Blot. Other applications not tested.

Recommended Dilution:
Immunohistochemistry: 1:25-1:100, with ABC as detection reagent
Western Blot: 1:50-1:500

Note: Use PBS buffer containing 0.1mM calcium chloride and 0.1mM magnesium chloride.
Optimum dilutions to be determined by researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG14A14Affinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Affinity purified 80 kD extracellular fragments of E-cadherin derived from tryptic digestion of A-431 human vulva carcinoma cells.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide. No stabilizing proteins added.
Specificity:
Recognizes human Cadherin E. Specific reactivity with 120kD E-cadherin protein and its 80kD trypsin-resistant extracellular part.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Moll R, MItze M, Frixen UH, Birchmeier W. Differential loss of E-Cadherin expression in infiltrating ductal and lobular breast carcinoma. Am J Pathol 143: 1731-1742 (1993).

2. Frixen UH, Behrens J, Sachs M, Eberle G, Voss B, Warda A, Löchner D, Birchmeier W. E-Cadherin – mediated cell – cell adhesion prevents invasiveness of human carcinoma cells. J Cell Biol 113: 173-185 (1991).

3. Umbas R, Schalken JA, Aalders TW, Carter BS, Karthaus HFM, Schaafsma HE, Debruyne FMJ, Isaacs WB. Expression of the cellular adhesion molecule E-Cadherin is reduced or absent in high-grade prostate cancer. Cancer Res 52: 5104-5109 (1992).