Technical Data
C0108-25P
CDH1, NT (E-Cadherin, Epithelial Cadherin, Uvomorulin, Cadherin-1, CAM 120/80, CD324, UVO)
Description:
CDH1 is a classical cadherin from the cadherin superfamily. This protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization.

Applications:
Suitable for use in Western Blot and Flow Cytometry. Other applications not tested.

Recommended Dilutions:
Flow Cytometry: 1:10-1:50
Western Blot: 1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
200ul-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
Synthetic peptide corresponding to a region betwen corresponding to a portion of amino acids within aa160-189 from the N-terminal region of human CDH1, conjugated to KLH.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Specificity:
Recognizes human CDH1 at ~97kD.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1.Mansouri,A., Differentiation 38(1), 67-71 (1988). 2. Knudsen,K.A. J. Cell Biol. 118(3), 671-679 (1992). 3. Hsu,Y.M., Cancer Res. 67(22), 11064-11073 (2007).