Technical Data
C0108-32
Cadherin E (Cadherin 1, E-Cadherin, L-CAM, Epithelial Cell Adhesion Molecule, Uvomorulin, CDH1)
Description:
E-cadherin (Uvomorulin, L-CAM, Epithelial Cell Adhesion Molecule, cell CAM 120/180) is a 123kD molecule of the cadherin family. One of the epithelial cell adhesion molecules, E-Cadherin, plays an important role in the formation of cell-cell contacts in epithelia irrespective their origin form ecto-, meso-or endodermal tissue. This early adhesion event between epithelial cells is a prerequisite for the assembly of all elements of the junctional complex. Furthermore, E-Cadherin plays a crucial role in the maintenance of the epithelial junctional complex and is as such an important molecule in maintaining epithelial integrity. Cadnerins constitute a familly of transmembrane glycoproteins involved in Ca2+ dependent cell-cell interactions. The members of this family are diferentially expressed in various tissues. One of the best characterized cadherins in E-cadherin, a 120 kD transmembrane glycoprotein consisting of an 80 kD extracellular and a 40kD transmembrane and cytoplasmic part. Cadherin E is a functional antibody which inhibits cell-cell adhesion. Antibodies against E-cadherin are suitable to characterize epithelial cells. E-Cadherin is involved in adhesion of epithelial cells (mostly homotypic and homophilic) and is expressed on immature erythroid cells. In addition, it blocks the interaction of E-cadherin molecules and therefore is suitable to study E-cadherin-mediated signal transduction.

Applications:
Suitable for use in Flow Cytometry, Western Blot, Functional blocking of E-cadherin. Not recommended for use in Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG12Q663Affinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
T-47D breast carcinoma line
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.6, 0.1% sodium azide.
Specificity:
Recognizes human Cadherin E at 123kD.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Buehring et al. (1996). Leukemia 10: 106.