Technical Data
Cadherin E (Cadherin 1, E-Cadherin, L-CAM, Epithelial Cell Adhesion Molecule, Uvomorulin, CDH1)
Cadherins are a multifunctional family of Ca 2+ -dependent, transmembrane glycoproteins, which promote and maintain cell adhesion in virtually all multicellular organisms. The cadherin superfamily comprises over forty proteins which are, on average, 5060% homologous (reviewed in ref 1). Cadherin expression is required for the assembly of cells into solid tissues and importantly, cadherins are expressed in a tissue speci c fashion (2) . Homotypic cellular interactions are promoted by homophillic interactions between the extracellular regions of like cadherin molecules on neighboring cells. Recent crystal structure analysis of an extracellular cadherin domain suggests that individual cadherin molecules cooperate to form a linear cell adhesion zipper.

Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1ug/ml
Immunoprecipitation: 25ug
Immunohistochemistry (formalin-fixed., paraffin embedded sections require a Heat Induced Epitope Retrieval (HIER) step prior to staining): 210ug/ml

Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
MabIgG1,k3G43Affinity Purified
100ug-20CBlue IceHumanMouse
The cytoplasmic domain of human E-cadherin expressed as a fusion protein with MBP.
Purified by Protein A affinity chromatography.
Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Speci c for E-cadherin but does show some minor crossreactivity with P-cadherin. Reacts with human E-Cadherin protein. Reactivity with other species has not been tested.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.