Technical Data
C0108-37J
Cadherin P
Description:
P-cadherin is a 120kD Ca2+-dependent cell adhesion molecule, which appears to be expressed in cells having high growth activity and/or high differentiation potential. In the early mouse embryo, P-cadherin has been detected in the extra-embryonic ectoderm, visceral endoderm, Iateral plate mesoderm, and notocord. In the late embryo, P-cadherin is detected in the placenta, epidermis and other epithelial tissues, the pigmented retina, and the mesothelium.
P-cadherin displays a rather unique distribution pattern in human tissues in that its expression is not detected in placental tissue. In humans, expression is restricted to epithelial tissues where it appears to be restricted to the basal or lower layers of strati ed epithelia.

Applications:
Suitable for use in Immunofluorescence, ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Also suitable as an inhibitor of P-cadherin dependent cell-cell contact. Other applications not tested.

Recommended Dilution:
Immuno uorescence: 2-5ug/ml
ELISA: 0.5ug/ml using immobilized A431 cells.
Western Blot (Native): 0.5-2ug/ml
Immunohistochemistry (FFPE): 2ug/ml; Requires heat induced epitope retrieval with citrate buffer, pH 6.0, prior to staining.
Inhibition of P-cadherin-dependent cell-cell contact: 100ug/ml
Optimal dilutions to be determined by the researcher.

Hybridoma:
Myeloma cell line P3-X63-Ag8-U1

Storage and Stability:

May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG13G51Highly Purified
SizeStorageShippingSourceHost
50ug4°C (-20°C Glycerol)Blue IceHumanMouse
Concentration:
~2mg/ml
Immunogen:
Epidermoid carcinoma cell line A431
Purity:
Puri ed by ammonium sulfate precipitation and anion exchange chromatography.
Form
Supplied as a liquid in TBS, 1% BSA, 40% glycerol. Recommended Diluent: 20mM TBS, pH 7.4, 2mM calcium chloride, 1% BSA.
Specificity:
Recognizes human P Cadherin.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
US Biological application reference: Figueira, E.C. et al., (2007) Investigative Ophthalmology and Visual Science. 48:144-156. 1. Fabricant, R.N., et al., Proc. Natl. Acad. Sci. 74: 565-569 (1977). 2. Shimoyama, Y., et al., Cancer Res. 49: 2128-2133 (1989). 3. Shmoyama, Y., et al., J. Cell Biol. 109: 1787-1794 (1989). 4. Shimoyama, Y., et al., Cancer Res. 51: 2185-2192 (1991). 5. Yasui, W., et al., Int. J. Cancer 54: 49-52 (1993). 6. Rasbridge, S.A., et al., J. Pathol. 169: 245-250 (1993). 7. Doki, Y., Cancer Res. 53: 3421-3426 (1993). 8. Morton, R.A., Cancer Res. 53: 3285-3590 (1993). 9. Glukhova, M., et al., Am. J. Pathology 146: 706-716 (1995). 10. Hodivala, K.J., Cell Biol. 124(4): 589-600 (1994).