Technical Data
C0108-37K
Cadherin P
Description:
Cadherins are a multifunctional family of Ca2+-dependent, transmembrane glycoproteins which promote and maintain cell adhesion in virtually all multicellular organisms. The cadherin superfamily comprises over forty proteins which are, on average, 50-60% homologous. Cadherin expression is required for the assembly of cells into solid tissues and importantly, cadherins are expressed in a tissue specific fashion. Homotypic cellular interactions are promoted by homophillic interactions between the extracellular regions of like cadherin molecules on neighboring cells. Recent crystal structure analysis of an extracellular cadherin domain suggests that individual cadherin molecules cooperate to form a linear cell adhesion zipper. In adherens junctions, cadherins are anchored to the actin cytoskeleton by interaction with the small cytoplasmic proteins beta-catenin and gamma-catenin which both bind to the actin binding protein alpha-catenin. In addition to playing important roles in differentiation and tissue morphogenesis, cadherins also appear to play a significant role in modulating tumor invasion and metastasis. The tissue specificity of cadherin subtypes is becoming valuable markers for the identification and differential diagnosis of certain cancers.

Applications:
Suitable for use in ELISA, Western Blot, Immunohistochemistry, Epitope mapping and Inhibition of P-cadherin-dependant cell-cell contact. Other applications have not been tested.

Recommended Dilutions:
Immunohistochemistry: 10ug/ml
Western Blot: 1-10ug/ml
Inhibition of P-cadherin-dependent cell-cell contact: 60ug/ml for adhesion blockage.
Optimal dilutions to be determined by the researcher.

Hybridoma: Myeloma cell line P3-X63-Ag8-U1

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2a3G52Highly Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceMouseRat
Concentration:
~0.5mg/ml (after reconstitution)
Immunogen:
Mouse endoderm cell line PSA5-E.
Purity:
Puri ed by ammonium sulfate precipitation and anion exchange chromatography.
Form
Supplied as a lyophilized powder from PBS, 1% BSA. No preservatives added. Reconstitute with 200ul sterile ddH2O. Dilute further in PBS, 1% BSA.
Specificity:
Recognizes mouse placental cadherin (P-cadherin).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
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