		Technical Data
C0114-01	Calcineurin, NT (Serine/Threonine Protein Phosphatase 2B Catalytic Subunit, alpha Isoform, Calmodulin-dependent Calcineurin A Subunit, alpha Isoform, CAM-PRP Catalytic Subunit, PPP3CA, CALNA, CNA)

Description:
PPP3CA is a calcium-dependent, calmodulin-stimulated protein phosphatase. This subunit may have a role in the calmodulin activation of calcineurin. PPP3CA dephosphorylates HSPB1 and SSH1.

Applications:
Suitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:1,000
Western Blot: 1:100-1:500
Immunohistochemistry: 1:50-1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Rabbit

Concentration:

~0.25mg/ml

Immunogen:

Synthetic peptide selected from the N-terminal region of human PPP3CA (KLH).

Purity:

Purified by ammonium sulfate precipitation.

Form

Supplied as a liquid in PBS, 0.09% sodium azide.

Specificity:

Recognizes human PPP3CA.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1.Rodriguez, A., et al., J. Biol. Chem. 280(11):9980-9984 (2005). 2.Shirane, M., et al., Nat. Cell Biol. 5(1):28-37 (2003). 3.Bengoechea-Alonso, M.T., et al., Nitric Oxide 8(1):65-74 (2003). 4.Huai, Q., et al., Proc. Natl. Acad. Sci. USA 99(19):12037-12042 (2002). 5.Bennasser, Y., et al., Virology 303(1):174-180 (2002).