Technical Data
C0114-02
Calcineurin A, pan (Protein Phosphatase 2B,PP2B, CnA)
Description:
Calcineurin, also known as protein phosphatase 2B (PP2B), is a calmodulin-dependent, calcium-activated, serine/threonine protein phosphatase composed of a catalytic subunit (calcineurin A) and a tightly bound regulatory subunit (calcineurin B) (1). Calcineurin A is highly homologous to protein phosphatases 1 and 2A. Calcineurin B, like calmodulin, contains four EF-hand, calcium-binding motifs.Calcineurin signaling has been implicated in a broad spectrum of cellular processes including cell-cycle regulation, stress response and apoptosis and is required for proper cardiovascular and skeletal muscle development (2,3). Calcineurin-mediated dephosphorylation of the nuclear factor of activated T cells (NFAT) transcription factor is essential for NFAT activation and nuclear translocation and early gene expression in T lymphocytes (2,3). Calcineurin is the target of the immunosuppressive drugs Cyclosporin A and FK506, both of which block the activation of quiescent T cells after T cell receptor engagement (2,3). Cyclosporin A and FK506 bind to the immunophilins, cyclophilin and FKBP respectively and the immunophilin-drug complex binds to calcineurin and blocks substrate binding.

Applications:
Suitable for use in Western Blot, Immunoprecipitation, Flow cytometry and Immunofluorescence. Other applications have not been tested.

Recommended Dilutions:
Western Blot: 1:1000
Immunoprecipitation:1:50
Immunofluorescence (IF-IC): 1:50
Flow Cytometry: 1:25
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanRabbit
Concentration:
Not determined
Immunogen:
Synthetic peptide (KLH-coupled) corresponding to the carboxy-terminus of human Calcineurin A (alpha isoform).
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol, 0.02% sodium azide.
Specificity:
Recognizes endogenous levels of Calcineurin A (alpha isoform). It may also recognize the beta and gamma isoforms of Calcineurin A. Does not cross-react with protein phosphatase 1 or 2A. Species Crossreactivity: human. mouse, rat, monkey, and D. melanogaster. Species sequence homology: chicken, bovine, xenopus and porcine.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1.Rusnak, F. and Mertz, P. (2000) Physiol. Rev. 80, 1483-521.Kahl, C.R. and Means, A.R. (2003) Endocr. Rev. 24, 719-36.Schulz, R.A. and Yutzey, K.E. (2004) Dev. Biol. 266, 1-16.