Technical Data
C0114-07A
Calcineurin A (Calcineurin-A, Calcineurin A alpha, CALNA, CALNA1, CALN, Calmodulin-dependent Calcineurin A Subunit alpha Isoform, CAM-PRP Catalytic Subunit, CCN1, CNA1, CNA-1, CNA, PPP2B, Protein Phosphatase 3 (formerly 2B) Catalytic Subunit alpha Isoform
Description:
Calcineurin is a calcium/calmodulin-dependent serine/threonine protein phosphatase. The active enzyme is a heterodimer consisting of a catalytic A subunit of 61kD and a myristylated regulatory B subunit of 19kD that binds calcium. The catalytic A subunit consists of an amino terminal catalytic domain, followed by calmodulin and B subunit binding sites, and a caboxyl-terminal autoinhibitory domain. Calnineurin is the target of immunosuppressant drugs such as cyclosporin A and FK506. Calcineurin controls the translocation of nuclear factors of activated T cells (NFAT), a family of transcriptional activators that control the expression of cytokine genes essential to immune response, from the cytosol into the nucleus of activated T cells. Calcineurin has been proposed to play an important role in mediating calcium-activated cell death.

Applications:
Suitable for use in Western Blot and Immunoprecipitation. Other applications have not been tested.

Recommended Dilutions:
Western Blot: 1-4 ug/ml
Immunoprecipitation: 10-20ug/ml
Optimal dilutions to be determined by the researcher.

Control Peptide: C0114-07A1

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanRabbit
Concentration:
~0.2mg/ml
Immunogen:
Synthetic peptide corresponding to residues surrounding aa267 of human Calcineurin A.
Purity:
Purified by immunoaffinitiy chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.5% BSA, 0.01% thimerosal, 30% glycerol.
Specificity:
Recognizes human Calcineurin A at ~61kD. A 32kD band can also be detected in human samples, presumably to be the cleavage product of Calcineurin A. Species Crossreactivity: mouse, rat, rabbit, bovine, hamster, dog.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.