Technical Data
Calcineurin B (CnB; Protein Phosphatase 2B, B subunit)
Calcineurin, also called Protein Phosphatase 2B, PP2B, PPP2B, Protein Phosphatase 3, and PPP3, is an enzyme that dephosphorylates serine and threonine residues in proteins. It is a heterodimer of a 59,000 dalton catalytic A subunit and a 1,900 dalton regulatory B subunit that is activated by the binding of calcium ions and calmodulin. Calcineurin is expressed in many tissues, but its levels are highest in the brain, where it may play a role in learning and memory. It has many substrates, including NFAT, a transcription factor that is activated by dephosphorylation. Complexes of the immunosuppressants cyclosporin and FK506 with immunophilin proteins such as cyclophilin and FKBP12 are potent and specific inhibitors of Calcineurin activity. Alterations in Calcineurin activity are suspected to play a role in cardiac hypertrophy and graft versus host disease in organ transplantation.

Suitable for use in Immunoprecipitation and Western Blot. Other applications have not been tested.

Recommended Dilution:
Western Blot: 0.5-2ug/ml. Detects Calcineurin B in rat brain preparation. Rat brain preparation was resolved by electrophoresis, transferred to nitrocellulose and probed with C0114-48 (0.5ug/ml). Proteins were visualized using a goat anti-rabbir secondary antibody conjugated to HRP and a chemiluminescence detection system.
Immunoprecipitation: 4ug immunoprecipitates Calcineurin B from 500ug of rat brain cytosol preparation.
Optimal dilutions to be determined by the researcher.

Recommended Secondary Antibodies:
I1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRb
I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb I1904-46Q: IgG, H&L (HRP) Pab Gt xRb

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
200ug-20CBlue IceHumanRabbit
Recombinant human Calcineurin B expressed in E. coli containing substitutions of Ala for Cys11 and Lys for Cys153.
Purified by ammonium sulfate precipitation.
Supplied as a liquid in 0.1M Tris-glycine, 0.15M sodium chloride, 0.05% azide, before the addition of glycerol to 30%.
Recognizes human Calcineurin B, Mr 19 kD. Species Crossreactivity: mouse, rat and bovine. Species sequence homology: Drosophila and yeast.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Klee, C.B., et al., J. Biol. Chem. 273: 1336713370, 1998. Ambach, A., et al., Eur. J. Immunol. 30: 34223431, 2000.