Technical Data
C0115-01C
Calcitonin Gene Related Peptide (CGRP)
Description:
Both neural and neuroendocrine cells are able to synthesize a variety of peptides as well as amino acids that can function either as inhibitory or stimulatory substances in neurotransmission. Thusfar, mostly polyclonal antibodies are available for many of them.

Hybridoma:
Derived from the hybridoma produced by fusion between myeloma cells and Balb/c spleen cells.

Applications:
Suitable for use in ELISA, Fluorescence Microscopy, and other immunohistochemical methods, works also on frozen sections if perfusion fixed. Paraformaldehyde must be included in the fixative with paraffin sections. Works also in Western Blot with BSA-conjugated samples. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20C or colder. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG12Q2268Affinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceHumanMouse
Concentration:
~0.1mg/ml
Immunogen:
A terminal fragment of CGRP-coupled to BSA with glutaraldehyde
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, 1% BSA and 0.1% sodium azide.
Specificity:
Highly specific to CGRP and does not react with other neuropeptides. Works at least on human and guinea pig tissues, but does not work on rat tissues.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Szabat, E., et al., Histochem. J. 26: 317-326 (1994).