Technical Data
C0115-01G
Calcitonin Gene-Related Peptide (CGRP)
Description:
Calcitonin gene-related peptide (CGRP) is present in C-cells of the thyroid and in central and peripheral nerves. CGRP has several important physiologic roles: it is a potent vasodilator, and can affect the force and rate of heart beat; it can modulate acetylcholine receptor function at the neuromuscular junction; it has been demonstrated to block tolerance to morphine; and it can modulate antigen presentation in Langerhans cells in the skin.

Applications:
Suitable for use in Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry: 1:100-1:500. Used on perfusion fixed tissue. Perfusion: 1) calcium-free Tyrodes solution, 2) paraformaldehyde-picric acid fixative, and 3) 10% sucrose in PBS as a cryo-protectant. Desired tissues were dissected and stored overnight in 10% sucrose in PBS. Slide-mounted tissue sections were processed for indirect immunofluorescence. Slides were incubated with blocking buffer for 1 hour at RT. C0115-01G was diluted with blocking buffer. Blocking buffer was removed from slides and they were then incubated for 18-24 hours at 4C with diluted C0115-01G. Slides were rinsed 3x and then incubated with secondary antibody for 1 hour at RT. Slides were again rinsed 3x and coverslipped. Staining was examined using fluorescence microscopy.
Optimal dilutions to be determined by the researcher.

Recommended Secondary Antibodies:
I1906: IgY, Chicken Pab Rb x Ch
I1906-10: IgY, Chicken-HRP Rb x Ch
I1906-12: IgY, Chicken-FITC Rb x Ch
I1906-14: IgY, Chicken-AP Rb x Ch

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgYSerum
SizeStorageShippingSourceHost
50ul-20CBlue IceRatChicken
Concentration:
Not Determined
Immunogen:
Synthetic peptide KDNFVPTNVGSEAF-NH2 corresponding to rat CGRP.
Purity:
Serum
Form
Supplied as a liquid, 0.05% sodium azide.
Specificity:
Recognizes rat CGRP.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.