Technical Data
C0115-01G
Calcitonin Gene-Related Peptide (CGRP)
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Technical Data |
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C0115-01G |
Calcitonin Gene-Related Peptide (CGRP) |
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Description: Calcitonin gene-related peptide (CGRP) is present in C-cells of the thyroid and in central and peripheral nerves. CGRP has several important physiologic roles: it is a potent vasodilator, and can affect the force and rate of heart beat; it can modulate acetylcholine receptor function at the neuromuscular junction; it has been demonstrated to block tolerance to morphine; and it can modulate antigen presentation in Langerhans cells in the skin. Applications: Suitable for use in Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunohistochemistry: 1:100-1:500. Used on perfusion fixed tissue. Perfusion: 1) calcium-free Tyrode’s solution, 2) paraformaldehyde-picric acid fixative, and 3) 10% sucrose in PBS as a cryo-protectant. Desired tissues were dissected and stored overnight in 10% sucrose in PBS. Slide-mounted tissue sections were processed for indirect immunofluorescence. Slides were incubated with blocking buffer for 1 hour at RT. C0115-01G was diluted with blocking buffer. Blocking buffer was removed from slides and they were then incubated for 18-24 hours at 4ºC with diluted C0115-01G. Slides were rinsed 3x and then incubated with secondary antibody for 1 hour at RT. Slides were again rinsed 3x and coverslipped. Staining was examined using fluorescence microscopy. Optimal dilutions to be determined by the researcher. Recommended Secondary Antibodies: I1906: IgY, Chicken Pab Rb x Ch I1906-10: IgY, Chicken-HRP Rb x Ch I1906-12: IgY, Chicken-FITC Rb x Ch I1906-14: IgY, Chicken-AP Rb x Ch Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
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