Description:
Cellular Localization: Secreted
Positive Control: Rat spinal cord.
Applications:
Suitable for use in Immunohistochemistry (Fr). Other applications not tested.
Recommended Dilution:
Immunohistochemistry (frozen): 1:2000-1:4000
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
|
| Type | Isotype | Clone | Grade |
|---|
| Pab | IgG | | Purified |
|
| Size | | Storage | Shipping | Source | Host |
|---|
| 100ul | | -20°C | Blue Ice | Rat | Sheep |
|
| Concentration: |
| Not determined |
| Immunogen: |
| Synthetic peptide (Rat) conjugated to BSA by a Glutaraldehyde linker. |
| Purity: |
| Purified |
| Form |
| Supplied as a liquid containing 0.1% sodium azide |
| Specificity: |
| On rat spinal cord immunostaining may be abolished by preincubation with 10nmol CGRP (rat alpha) per mL diluted antiserum. Neither substance P nor galanin have any effect on immunostaining when used at 10nmol/mL diluted antiserum. It is believed that this antibody crossreacts with all known forms of rat and human CGRP. Species Crossreactivity: Crossreacts with Human and Rat. |
|
| Intended for research use only. Not for use in human, therapeutic, or diagnostic applications. |
Li YJ et al. Calcitonin gene-related peptide-induced preconditioning protects against ischemia-reperfusion injury in isolated rat hearts. Eur J Pharmacol 311:163-7 (1996). Amara SG et al. Expression in brain of a messenger RNA encoding a novel neuropeptide homologous to calcitonin gene-related peptide. Science 229:1094-7 (1985). Gibson SJ et al. Calcitonin gene-related peptide immunoreactivity in the spinal cord of man and of eight other species. J Neurosci 4:3101-11 (1984). Amara SG et al. Alternative RNA processing in calcitonin gene expression generates mRNAs encoding different polypeptide products. Nature 298:240-4 (1982).
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