Cellular Localization: Secreted
Positive Control: Rat spinal cord.
Suitable for use in Immunohistochemistry (Fr). Other applications not tested.
Immunohistochemistry (frozen): 1:2000-1:4000
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
| Not determined|
|Synthetic peptide (Rat) conjugated to BSA by a Glutaraldehyde linker.|
|Supplied as a liquid containing 0.1% sodium azide|
|On rat spinal cord immunostaining may be abolished by preincubation with 10nmol CGRP (rat alpha) per mL diluted antiserum. Neither substance P nor galanin have any effect on immunostaining when used at 10nmol/mL diluted antiserum. It is believed that this antibody crossreacts with all known forms of rat and human CGRP. Species Crossreactivity: Crossreacts with Human and Rat. |
|Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.|
Li YJ et al. Calcitonin gene-related peptide-induced preconditioning protects against ischemia-reperfusion injury in isolated rat hearts. Eur J Pharmacol 311:163-7 (1996). <8891596>Amara SG et al. Expression in brain of a messenger RNA encoding a novel neuropeptide homologous to calcitonin gene-related peptide. Science 229:1094-7 (1985). <2994212>Gibson SJ et al. Calcitonin gene-related peptide immunoreactivity in the spinal cord of man and of eight other species. J Neurosci 4:3101-11 (1984). <6209366>Amara SG et al. Alternative RNA processing in calcitonin gene expression generates mRNAs encoding different polypeptide products. Nature 298:240-4 (1982). <6283379>|