Technical Data
C0115-08B
Calcitonin
Description:
Calcitonin is a 32aa peptide which can be demonstrated in C cells of the normal and hyperplastic thyroid. Staining for calcitonin may be used for the identification of a spectrum of C cell proliferative abnormalities ranging from C cell hyperplasia to invasive tumors. Staining for calcitonin in medullary carcinoma of the thyroid produces a fine granular pattern in the cytoplasm. Amyloid deposits within the tumor may also exhibit varying degrees of calcitonin activity.

Applications:
Suitable for use in Immunohistochemistry. Other aplications not tested.

Recommended Dilutions:
Immunohistochemistry (Formalin/paraffin): 1:200 for 30 minutes at RT. No special pretreatment is required for the immunohistochemical staining of formalin/paraffin tissues. Optimal dilutions to be determined by the researcher.

Recommended Positive Control:
Thyroid or medullary carcinoma

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG5G25Supernatant
SizeStorageShippingSourceHost
500ul-20CBlue IceHumanRabbit
Concentration:
Not Determined
Immunogen:
Synthetic peptide corresponding to aa1-32 of human calcitonin. Cellular Localization: Cytoplasmic.
Purity:
Supernatant
Form
Supplied as a liquid, 15mM sodium azide.
Specificity:
Recognizes human Calcitonin. Species Crossreactivity: monkey, mouse, rat, canine, equine, sheep and porcine.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Spieker-Polet, H., et al., Proc. Natl. Acad. Sci. 92: 9348-9352 (1995). 2. Liguori, M.J., et al., Hybridoma 20: 189-198 (2001). 3. Cano, G., et al., Diagn. Cytopathol. 29: 207-211 (2003). 4. Diaz, L.K. & Sneige, N., Adv. Anat. Pathol. 12: 10-19 (2005). 5. Huang, Z., et al., Appl. Immunohistochem. Mol. Morphol. 13: 91-95 (2005). 6. Rossi, S., et al., Modern Pathology 17(1): 361A (2004). 7. Blechner, M., et al., Modern Pathology 17(1): 241A (2004). 8. Cheuk, W., et al., Am. J. Surg. Path. 28: 801-807 (2004). 9. Budd, G.B., et al., Abstract presented at ASCO Annual meeting, June 2004, New Orleans. 10. Tarr, S.M., et al., Abstract presented at Association for Molecular Pathology meeting, Los Angeles, 2004. 11. Gown, A.M., et al., Modern Pathology 18(1): 35A (2005). 12. Treaba, D.O., et al., Modern Pathology 18(1): 53A (2005). 13. Rossi, S., et al., Am. J. Clin. Pathol. 124: 295-302 (2005).