Technical Data
Calcitonin is a 32 amino acid peptide which can be demonstrated in C cells of the normal and hyperplastic thyroid. Staining for calcitonin may be used for the identification of a spectrum of C cell proliferative abnormalities ranging from C cell hyperplasia to invasive tumors. Stining for calcitonin in medullary carcinoma of the thyroid produces a fine granular pattern in the cytoplasm. Amyloid deposits within the tumor may also exhibit varying degrees of calcitonin activity.

Suitable for use in Immunohistochemistry. Other applications have not been tested.

Recommended Dilution:
Immunohistology (Formalin/paraffin): 1:150 for 10 min at RT Note: Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
Optimal dilutions to be determined by the researcher.

Positive Control:
Thyroid or medullary carcinoma

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabAffinity Purified
250ul4C (-20C Glycerol)Blue IceHumanRabbit
Synthetic human calcitonin peptide (aa1-32). Cellular Localization: Cytoplasmic.
Purified by immunoaffinity chromatography.
Supplied as a liquid in 10mM PBS, pH 7.6, 0.2% BSA and 15mM sodium azide, before the addition of glycerol to 40%.
Recognizes human Calcitonin. Species Crossreactivity: Human, monkey, mouse, rat, canine, equine, sheep and porcine.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.