Technical Data
C0115-08C
Calcitonin
Description:
Calcitonin is a 32 amino acid peptide which can be demonstrated in C cells of the normal and hyperplastic thyroid. Staining for calcitonin may be used for the identification of a spectrum of C cell proliferative abnormalities ranging from C cell hyperplasia to invasive tumors. Stining for calcitonin in medullary carcinoma of the thyroid produces a fine granular pattern in the cytoplasm. Amyloid deposits within the tumor may also exhibit varying degrees of calcitonin activity.

Applications:
Suitable for use in Immunohistochemistry. Other applications have not been tested.

Recommended Dilution:
Immunohistology (Formalin/paraffin): 1:150 for 10 min at RT Note: Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
Optimal dilutions to be determined by the researcher.

Positive Control:
Thyroid or medullary carcinoma

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabAffinity Purified
SizeStorageShippingSourceHost
250ul4°C (-20°C Glycerol)Blue IceHumanRabbit
Concentration:
~0.1mg/ml
Immunogen:
Synthetic human calcitonin peptide (aa1-32). Cellular Localization: Cytoplasmic.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in 10mM PBS, pH 7.6, 0.2% BSA and 15mM sodium azide, before the addition of glycerol to 40%.
Specificity:
Recognizes human Calcitonin. Species Crossreactivity: Human, monkey, mouse, rat, canine, equine, sheep and porcine.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.