Technical Data
C0115-08J
Calcitonin (CALCA, CALC1)
Description:
The calcitonin family of bioactive peptides comprises of calcitonin, amylin, two calcitonin-gene related peptides (CGRP1, and CGRP2) and adrenomedullin (ADM). Calcitonin is 32aa peptide found in the parafollicular "C" cells of the thyroid in mammals. It is also found in a number of non-mammals. It regulated the mineral (calcium and phosphate) balance. Calcitonin causes hypercalcemia by acting as an inhibitor of osteoclast induced bone resorption.

The calcitonin family peptides probably act through G-protein coupled membrane receptors. Recently, a homolog of calcitonin receptor, CRLR (calcitonin-receptor-like receptor human 461aa; rat/mouse 463aa) was identified. It is now shown that CRLR can function as either a CGRP receptor or an ADM receptor, depending upon which members of a new family of proteins called receptor activity modifying proteins (RAMP1-3) are expressed.

Applications:
Suitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 0.1-1ug/ml
Immunohistochemistry: 2:20ug/ml
Western Blot: 1-10ug/ml (ECL)
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG111C94Affinity Purified
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanMouse
Concentration:
As reported
Immunogen:
Human calcitonin full length Calcitonin/CALC; location ~N- terminus (Accession #P01258)
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
Specificity:
Recognizes human Calcitonin. Crossreactivity: human pro-calcitonin.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Le Moullec JM et al (1984) FEBS lett. 167,93. 2. Nelkin, BD et al (1984) BBRC 1123, 648. 3. Edbrooke MR et al (1985) EMBo J. 4, 715-724. 4. Jonas V et al (1985) PNAS 82, 1994. 5. Riley, JH et al (1986) FEBS lett. 198, 71. 6. Broad PM et al (19890 Nucl. Acid. Res. 17, 6999. 7. Bracq S et al (1993) FEBS Lett. 331, 15. 8. McLatchie LM et al (1998) Nature 393, 333-339.