		Technical Data
C0115-08V	Calcitonin (CALC1, CALCA, CGRP, CGRP1, CGRP-I, CT, KC)

Description:
Calcitonin is found in C cells of normal and hyperplastic thyroid. This antibody can be used to detect the presence of calcitonin in these tissues.

Applications:
Suitable for use in Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry: 1:100-1:200 for 1 hr. at RT
Optimal dilutions to be determined by the researcher.

Positive Control:
C cells of thyroid, cytoplasmic staining pattern.

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Pab	IgG		Purified

Size		Storage	Shipping	Source	Host
0.5ml		-20°C	Blue Ice	Human	Rabbit

Concentration:

Not determined

Immunogen:

Synthetic peptide corresponding to aa1-32 of human Calcitonin coupled to BSA.

Purity:

Purified immunoglobulin fraction.

Form

Supplied as a lyophilized powderin PBS, 1% BSA, 15mM sodium azide. Reconstitute with 0.5ml dH2O.

Specificity:

Recognizes human Calcitonin. Species Crossreactivity: Canine, equine, feline, mouse, rat, sheep and swine.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1.Holm R, Nesland J M, Attramadal A, et al.. Null cell adenomas of the pituitary gland. An immunohistochemical study. J. Path. 158:213-217 (1989). 2.Talerman A, Lindeman J, Kievit-Tyson P A, et al.. Demonstration of cacitonin and carcinoembryonic antigen (CEA) in medullary carcinoma of the thyroid (MCT) by immunoperoxidase technique. Histopathology. 3:503-510 (1979).