Description:
Cellular Localization: Cytoplasmic
Applications: Suitable for use in RID, Immunofluorescence, ELISA and Western Blot. Other applications not tested.
Recommended Dilution: Optimal dilutions to be determined by the researcher.
Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| Type | Isotype | Clone | Grade |
| Mab | IgG1 | 8J480 | |
|
| Size | | Storage | Shipping | Source | Host |
| 100ul | | -20°C | Blue Ice | Chicken | Mouse |
|
| Concentration: |
| As reported |
| Immunogen: |
| Purified chicken gizzard caldesmon. Epitope: The antibody is directed to an epitope near the carboxy-terminusCa2+Calmodulin and F-actin binding site of caldesmon. |
| Purity: |
| Ascites |
| Form |
| 0.1% Sodium Azide; Raw Ascites |
| Specificity: |
| Using immunoblot techniques Monoclonal anti-Caldesmon localizes both the high molecular weight form and the low molecular weight non-muscle form of caldesmon. Monoclonal anti-Caldesmon localizes the high molecular weight form of caldesmon in porcine stomach and turkey gizzard, and the low molecular weight non-muscle caldesmon in gerbil fibroma cells (CCL-146), normal rat kidney cells (NRK), human EJ and Hut-11 cells and chicken embryo fibroblast cells. Species Crossreactivity: Crossreacts with Human, Rat, Gerbil, porcine and Turkey.Not yet tested in other species. |
|
| Intended for research use only. Not for use in human, therapeutic, or diagnostic applications. |
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