Technical Data
C0132-14F
Caldesmon, smooth
Description:
Caldesmon is an actin and myosin binding protein. It plays a major role in the regulation of smooth muscle and non-muscle contractile events. At least 5 isoforms are known from alternate splicing. High molecular weight h-caldesmon (isoform 1) of 120-150kD is predominantly expressed in smooth muscles. Lower molecular weight caldesmon l-caldesmon (isoforms 2,3,4 and 5) of 61-70kD is more abundant in non-muscle tissues and cells. This antibody reacts specifically with h-caldesmon

Applications:
Suitable for use in Immunohistochemistry and Western Blot . Other applications not tested.

Recommended Dilution:
Western Blot: 1-2 ug/ml
Immunohistochemistry (formalin, paraffin): 2.0-4.0ug/ml.
Optimal dilution determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at 4C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
Mab9G326Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
As reported
Immunogen:
Human uterus smooth muscle extract.
Purity:
Purified by affinity chromatography.
Form
Supplied as a lyophilized powder from 1.2% sodium acetate, 2% BSA, 0.2% sodium azide. Reconstitute with sterile PBS to concentration of 0.1-1mg/ml.
Specificity:
Recognizes human caldesmon. Species Crossreactivity: mouse; rat
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.