		Technical Data
C0132-14F	Caldesmon, smooth

Description:
Caldesmon is an actin and myosin binding protein. It plays a major role in the regulation of smooth muscle and non-muscle contractile events. At least 5 isoforms are known from alternate splicing. High molecular weight h-caldesmon (isoform 1) of 120-150kD is predominantly expressed in smooth muscles. Lower molecular weight caldesmon l-caldesmon (isoforms 2,3,4 and 5) of 61-70kD is more abundant in non-muscle tissues and cells. This antibody reacts specifically with h-caldesmon

Applications:
Suitable for use in Immunohistochemistry and Western Blot . Other applications not tested.

Recommended Dilution:
Western Blot: 1-2 ug/ml
Immunohistochemistry (formalin, paraffin): 2.0-4.0ug/ml.
Optimal dilution determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Mab		9G326	Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Mouse

Concentration:

As reported

Immunogen:

Human uterus smooth muscle extract.

Purity:

Purified by affinity chromatography.

Form

Supplied as a lyophilized powder from 1.2% sodium acetate, 2% BSA, 0.2% sodium azide. Reconstitute with sterile PBS to concentration of 0.1-1mg/ml.

Specificity:

Recognizes human caldesmon. Species Crossreactivity: mouse; rat

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.