Technical Data
Caldesmon, phosphorylated (Ser759) (CDM, Caldesmon 1, Caldesmon-1, CALD1, H CAD, H-CAD, L-CAD)
Caldesmon is a smooth muscle and nonmuscle regulatory protein that interacts with actin, myosin, tropomyosin, and calmodulin (1,2). Smooth muscle caldesmon is an elongated molecule with a calmodulin, tropomyosin, and actin-binding region at the C-terminus and a
myosin-binding domain at the N-terminus (3). Caldesmon stimulates actin binding of tropomyosin which increases the stabilization of actin filament structure. In muscle tissues,
caldesmon inhibits the actomyosin ATPase by binding to F- actin. This inhibition is reduced by calcium-calmodulin and is promoted by tropomyosin (4).Phosphorylation of caldesmon
by extracellular signal-regulated kinase (ERK) and p38 mitogen-activated protein (MAP) kinases in smooth muscle are important for actin and tropomyosin binding, and actomyosin inhibitory activity.In intact vascular smooth muscle Caldesmon is phosphorylated by proline-directed
protein kinases, members of the MAP kinase family, suggesting that caldesmon phosphorylation by MAP kinase may modulate smooth muscle contraction (5).

Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1500-1:3000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
100ul-20°CBlue IceHumanRabbit
Not determined
A phospho-specific peptide corresponding to residues surrounding Serine 759 of human Caldesmon.
Supplied as a liquid in 50mM Tris-glycine, pH 7.4, 0.15M sodium chloride, 40% glycerol, 0.01% sodium azide, 0.05% BSA.
Recognizes human Caldesmon when phosphorylated Ser759.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Humphrey, M.B., et al. Gene 112: 197–204 (1992). 2. Bryan, J., et al. J Biol Chem. 264: 13873–9 (1989). 3. Bryan, J., et al. Ann N Y Acad Sci. 599: 100–10 (1990). 4. Fraser, I.D., et al. Biochemistry 36: 5483–92 (1997). 5. Hedges J, et al. Am J Physiol Cell Physiol 278: C718- C726, 2000