Technical Data
C0132-15C
Caldesmon, phosphorylated, Ser789
Description:
Caldesmon is a smooth muscle and nonmuscle regulatory protein that interacts with actin, myosin, tropomyosin, and calmodulin (1,2). Smooth muscle caldesmon is an elongated molecule with a calmodulin, tropomyosin, and actin-binding region at the C-terminus and a myosin-binding domain at the N-terminus (3). Caldesmon stimulates actin binding of tropomyosin which increases the stabilization of actin filament structure. In muscle tissues, caldesmon inhibits the actomyosin ATPase by binding to F-actin. This inhibition is reduced by calcium-calmodulin and is promoted by tropomyosin (4).

Applications:
Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested.

Recommended Dilutions:
Flow Cytometry: 1:20
Western Blot: 1:10,000-1:20,000
Immunohistochemistry: 1:250
Immunocytochemistry: 1:250
Immunoprecipitation: 1:80
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Manufactured incorporating RabMAb technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
TypeIsotypeCloneGrade
MabIgG6k64Supernatant
SizeStorageShippingSourceHost
100ul4C (-20C Glycerol)Blue IceHumanRabbit
Concentration:
Not Determined
Immunogen:
A synthetic phospho-peptide corresponding to residues surrounding Ser789 of human Caldesmon.
Purity:
Supernatant
Form
Supplied as a liquid in 50mM Tris-Glycine, pH 7.4, 0.15M sodium chloride, 0.01% sodium azide, 0.05% BSA , 40% glycerol.
Specificity:
Recognizes human Caldesmon. This antibody is not phospho-specific, and will recognize total endogenous protein. Species Crossreactivity: mouse and rat
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Humphrey, M.B., et al. Gene 112: 197204 (1992).2. Bryan, J., et al. . J Biol Chem. 264: 138739 (1989). 3. Bryan, J. Ann N Y Acad Sci. 599: 10010 (1990). 4. Fraser, I.D., et al. Biochemistry 36: 548392 (1997).