Technical Data
C0132-15D
CALD1, ID (Caldesmon, CDM, CAD, CALD1)
Description:
CALD1 is a calmodulin- and actin-binding protein that plays an essential role in the regulation of smooth muscle and nonmuscle contraction. The conserved domain of this protein possesses the binding activities to Ca(2+)-calmodulin, actin, tropomyosin, myosin, and phospholipids. This protein is a potent inhibitor of the actin-tropomyosin activated myosin MgATPase, and serves as a mediating factor for Ca(2+)-dependent inhibition of smooth muscle contraction.

Applications:
Suitable for use in ELISA, Flow Cytometry, Immunohistochemistry and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:1000
Flow Cytometry: 1:10-1:50
Immunohistochemistry: 1:10-1:50
Western Blot: 1:50-1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGPurified
SizeStorageShippingSourceHost
200ul-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
KLH-conjugated synthetic peptide corresponding to a portion of amino acids within aa435-465 from the center region of human CALD1 (Accession #Q05682).
Purity:
Purified by ammonium sulfate precipitation.
Form
Supplied as a liquid in PBS, 0.09% sodium azide
Specificity:
Recognizes human CALD1.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Yoshio,T., FEBS Lett. 581(20), 3777-3782 (2007). 2. Mani,R.S., Biochemistry 31(47), 11896-11901 (1992).