Technical Data
C1032-40X
Caldesmon, LMW (BSA & Azide Free)
Description:
Caldesmon is a developmentally regulated protein involved in smooth muscle and non-muscle contraction. Two closely related variants of human caldesmon have been identified which differ in their eletrophoretic mobility and cellular distribution. The h-caldesmon variant (120-150kD) is predominantly expressed in smooth muscle whereas l-caldesmon (70-80kD) is found in nonmuscle tissue and cells. Neither of the two variants has been detected in skeletal muscle.

MW of Antigen: 80kD (l-caldesmon)

Epitope: Not determined

Applications and Recommended Dilutions:
Immunofluorescence
Western Blotting (Ab 1-2ug/ml for 2hrs at RT)
Immunohistochemistry (Formalin/paraffin) (Use Ab at 1-2ug/ml for 30 min at RT) * [Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.] The optimal dilution for a specific application should be determined by the investigator.

Positive Control: Rh30 cells. Uterus

Cellular Localization: Cytoplasmic

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at-20C or colder. Aliquots are stable for at least 12 months at-20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG1,k3F41Affinity Purified
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Recombinant caldesmon
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in 10mM PBS, pH 7.4. Also available with BSA and azide. See C1032-40
Specificity:
Recognizes Caldesmon, LMW . Species Crossreactivity: human.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Warren et al., J. Cell Biol. 125:359368 (1994).