		Technical Data
C1033	Caldesmon (Ser789), phosphorylated

Description:
Applications:
Suitable for use in Kinase assay and Western Blot. Other applications have not been tested.

Recommended Dilution:
Kinase Assay: 0.5-2ug/ml. Active MAP Kinase 2/Erk2 (Catalog #M2362-06) was used to phosphorylate serine 789 of purified porcine (hog) stomach Caldesmon.
Western Blot: 2ug/ml. Detects phosphorlyated isoform of Caldesmon in a RIPA lysate of EGF stimulated human A431 cells. Pretreating blots with 40U/ml of lambda phosphatase prior to incubation abolished the detection of Caldesmon.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Rabbit

Concentration:

~0.3mg/ml

Immunogen:

Synthetic corresponding to amino acids 782-793 (C-QSVDKVT[ pS]PTKV) of human Caldesmon, KLH-conjugated. The immunizing sequence is identical in the rat.

Purity:

Purified by Protein A affinity chromatography.

Form

Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, before the addition of glycerol to 30%.

Specificity:

Recognizes human Caldesmon phosphorylated at Ser789 at ~Mr 140kD along with several lower molecular weight isoforms. Species Crossreactivity: porcine, canine and ferret. Species sequence homology: rat

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. D’Angelo, G., et al., J. Biol. Chem. 274: 30115-30121,1999. 2. Hedges, J.C., et al., Amer. J. Physiol. 278: C718-C726, 2000. 3. Sobue, K. and J. R. Sellers, J. Biol. Chem. 266: 12115-12118, 1991.