Technical Data
C1033-51Y
Calgranulin A, B (Calgranulin-A, CAGA, CGLA, Calgranulin-B, CAGB, CGLB, 60B8AG, Calprotectin L1L Subunit, Calprotectin L1H Subunit, CP-10, Cystic Fibrosis Antigen, CFAG, L1Ag, Leukocyte L1 Complex Light Chain, Leukocyte L1 Complex Heavy Chain, LIAG, MA387
Description:
The calcium binding, migration inhibitory factor-related proteins, MRP-8 (S100A8) and MRP-14 (S100A9) belong to the S100 protein family. The expression of these proteins is largely confined to the cytosol of neutrophils and monocytes. The complex formation of these proteins is a calcium-dependent process. The S100A8/A9 heterocomplex, also called MRP-8/MRP-14 complex or calprotectin, comprises 60% of the cytoplasmic protein fraction of circulating polymorphonuclear granulocytes and is also found in monocytes, macrophages and ileal tissue eosinophils. Peripheral blood monocytes carry the antigen extra- and intracellularly, neutrophils only intracellularly. The S100A8/A9 complex has antibacterial, antifungal and immunomodulating and antiproliferative effects. Besides this it is a potent chemotactic factor for neutrophils. Plasma concentrations are elevated in diseases associated with increased neutrophil activity. Granulocytes terminate their existence after transmigration through the intestinal wall. Therefore calprotectin is also detectable in faeces. The monoclonal antibody 27E10 can be used for early detection of inflammatory macrophages, for the characterisation of tumorous tissues and the monitoring of peripheral blood cell cultures.

Applications:
Suitable for use in Western Blot, Immunoprecipitation and Flow Cytometry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 1:10.
Western Blot: 1:10
Immunoprecipitation: 1:10.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG127E10Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceHumanMouse
Concentration:
~0.1mg/ml
Immunogen:
Human Calgranulin A, B.
Purity:
Purified
Form
Supplied as a liquid in 0.02% sodium azide, 0.1% BSA.
Specificity:
Recognizes an epitope on the S100A8/A9 heterocomplex that is not exposed on the individual subunits S100A8 (MRP8) or S100A9 (MRP14). Does not react with lymphocytes or platelets. Shows cross-reaction with a subpopulation of macrophages of rhesus monkey.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Zwadlo, G et al; A monoclonal antibody to a subset of human monocytes found only in the peripheral blood and inflammatory tissues. J Immunol 1986, 137: 512 2. Kuhn, A et al; Upregulation of epidermal surface molecule expression in primary and ultraviolet-induced lesions of lupus erythematosus tumidus. Br J Dermatol 2002, 146: 801 3. Hessian, P et al; The heterodimeric complex of MRP-8 (S100A8) and MRP-14 (S100A9) Antibody recognition, epitope definition and the implications for structure. Eur J Biochem 2001, 268: 353.