Technical Data
C1035-01C
Calmodulin (CALM 1, CALM 2, CALM 3, CALM, CALM1, CALM2, CALM3, CALML2, CAM 1, CAM 2, CAM 3, CAM I, CAM1, CAM2, CAM3, CAMB, CAMC, CAMI, CAMIII, CaM, Calmodulin 1, Calmodulin1, DD132, PHKD, Phosphorylase kinase delta, Phosphorylase kinase delta subunit)
Description:
Calmodulin is a small, highly conserved calcium binding protein found in all eukaryotic cells. With the capacity to bind up to four calcium ions, this 17kD protein acts as an important intracellular receptor for regulatory calcium signals. As it binds calcium, calmodulin undergoes conformational changes which can increase its affinity for target proteins. It acts both directly, through interaction with key target enzymes, and indirectly, via specific kinases. Studies have found that calmodulin participates in the regulation of several biological processes including energy and biosynthetic metabolism, cell motility, exocytosis, cytoskeletal assembly, and intracellular modulation of both cAMP and calcium concentrations.

Applications:
Suitable for use in ELISA, Western Blot and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: Detects a 17kD protein representing calmodulin from Dictyostelium cell lysate.
Optimal dilutions to be determined by the researcher.

Storage and Stability:

May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG18J483Ascites
SizeStorageShippingSourceHost
200ul-20CBlue IceMouse
Concentration:
Not determined
Immunogen:
Calmodulin purified from Dictyostelium discoideum.
Purity:
Ascites
Form
Supplied as a liquid in PBS, pH 7.2, 0.05% sodium azide.
Specificity:
This antibody detects calmodulin. It does not detect parvalbumin, tropinin, S-100, or myosin light chain kinase (MLCK). Species Crossreactivity: Crossreacts with bovine, chicken, E. coli, rat, Chlamydomonas reinhardtii and Dictyostelium discoideum.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
This product has been used in:Hulen D et al. Production and specificity of monoclonal antibodies against calmodulin from Dictyostelium discoideum. Cell Motil Cytoskeleton 18:113-22 (1991). Zhu Q et al. Calmodulin and the contractile vacuole complex in mitotic cells of Dictyostelium discoideum. J Cell Sci 104 (Pt 4):1119-27 (1993). Zhu Q & Clarke M Association of calmodulin and an unconventional myosin with the contractile vacuole complex of Dictyostelium discoideum. J Cell Biol 118:347-58 (1992). Coling DE & Salisbury JL Characterization of the calcium-binding contractile protein centrin from Tetraselmis striata (Pleurastrophyceae). J Protozool 39:385-91 (1992). Clarke M et al. Density-dependent induction of discoidin-I synthesis in exponentially growing cells of Dictyostelium discoideum. Differentiation 34:79-87 (1987).