Technical Data
C1035-01D
Calmodulin (CALM 1, CALM 2, CALM 3, CALM, CALM1, CALM2, CALM3, CALML2, CAM 1, CAM 2, CAM 3, CAM I, CAM1, CAM2, CAM3, CAMB, CAMC, CAMI, CAMIII, CaM, Calmodulin 1, Calmodulin1, DD132, PHKD, Phosphorylase kinase delta, Phosphorylase kinase delta subunit)
Description:
Calmodulin is a small, highly conserved calcium binding protein found in all eukaryotic cells. With the capacity to bind up to four calcium ions, this 17kD protein acts as an important intracellular receptor for regulatory calcium signals. As it binds calcium, calmodulin undergoes conformational changes which can increase its affinity for target proteins. It acts both directly, through interaction with key target enzymes, and indirectly, via specific kinases. Studies have found that calmodulin participates in the regulation of several biological processes including energy and biosynthetic metabolism, cell motility, exocytosis, cytoskeletal assembly, and intracellular modulation of both cAMP and calcium concentrations.

Positive Control: Dictyostelium cell lysate.

Applications:
Suitable for use in ELISA, Western Blot and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG18J484Ascites
SizeStorageShippingSourceHost
200ul-20CBlue IceMouse
Concentration:
Not determined
Immunogen:
Calmodulin purified from Dictyostelium discoideum.
Purity:
Ascites
Form
0.05% Sodium Azide; PBS diluted Ascites
Specificity:
This antibody does not detect parvalbumin, tropinin, S-100, or myosin light chain kinase (MLCK). Species Crossreactivity: Crossreacts with Chlamydomonas, Dictyostelium, Chicken, bovine and Rat. Not yet tested in other species.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Hulen D et al. Production and specificity of monoclonal antibodies against calmodulin from Dictyostelium discoideum. Cell Motil Cytoskeleton 18:113-22 (1991). Clarke M et al. Density-dependent induction of discoidin-I synthesis in exponentially growing cells of Dictyostelium discoideum. Differentiation 34:79-87 (1987).