Technical Data
C1035-04D
Calmodulin Kinase II, phosphorylated (Thr 286)
Description:
Calcium/calmodulin kinase II (CaM Kinase II) is a multi-functional calcium and calmodulin-dependent protein kinase that mediates cellular responses to second messengers. The antibody was raised against a synthetic phosphopeptide corresponding to amino acid residues surrounding the phosphorylated-Thr286 found in rat brain CaM Kinase II. Phosphorylation of Thr286 produces an autonomously active form of CaM Kinase II.

Applications:
Specific for the detection of CaM Kinase II phosphorylated at Thr286 protein in Western blot and Dot blot. Not tested in other applications.

Recommended Dilution:
Western Blot: 1:500 to 1:2000.
Dot Blot: 1:500 to 1:2000.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ul-20CBlue IceRatRabbit
Concentration:
Not determined
Immunogen:
Synthetic phosphopeptide corresponding to amino acid residues surrounding the phosphorylated-Thr286 found in rat brain CaM Kinase II.
Purity:
Purified by sequential chromatography on a Protein A column and phospho- and non-phospho-peptide affinity columns.
Form
Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol.
Specificity:
Recognizes rat Calmodulin Kinase II when phosphorylated at Thr 286. Species crossreactivity: mouse, human, Xenopus.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Kennedy, M. B., Signal-processing machines at the postsynaptic density, Science, 290 (2000) 750 754.