Technical Data
C1035-22F
CAMK1 (Calcium/calmodulin-dependent protein kinase I, CaMKI, CAMKI, CaM-KI, CaMKI-alpha, CaM kinase I, CaM kinase I alpha, MGC120317, MGC120318)
Description:
Calcium/calmodulin-dependent protein kinase I is expressed in many tissues and is a component of a calmodulin-dependent protein kinase cascade. Calcium/calmodulin directly activate calcium/calmodulin-dependent protein kinase I by binding to the enzyme and indirectly promotes the phosphorylation and synergistic activation of the enzyme by calcium/calmodulin-dependent protein kinase I kinase.

Applications:
Suitable for use in Western Blot, Immunocytochemistry and Immunohistochemistry (Paraffin). Other applications not tested.

Recommended Dilution:
Western Blot: 1:5000-10000
Immunohistochemistry: 1:100-250 (Paraffin)
Immunocytochemistry: 1:100-250
Optimal dilutions to be determined by the researcher.

Positive Control:
Human brain, mouse brain, rat brain, and SHSY5Y lysates.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG10F633Supernatant
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanRabbit
Concentration:
Not determined
Immunogen:
Synthetic peptide corresponding to residues on the N-terminus of human Calcium/Calmodulin Kinase I.
Purity:
Supernatant
Form
Supplied as a liquid in PBS 50mM Tris-Glycine, pH 7.4, 0.15M NaCl, 40% glycerol, 0.01% sodium azide, 0.05% BSA.
Specificity:
Recognizes human Calcium/Calmodulin Kinase I. Species crossreactivity: mouse, rat. Sequence homology: mammals.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.