Technical Data
C1035-22G
CaM Kinase IB, CT (R331) (CaM Kinase I beta, Calcium/Calmodulin-dependent Protein Kinase Type 1B, CaM-KI beta, CaMKI-beta, Pregnancy Up-regulated Non-ubiquitously-expressed CaM Kinase, PNCK)
|
Technical Data |
|
C1035-22G |
CaM Kinase IB, CT (R331) (CaM Kinase I beta, Calcium/Calmodulin-dependent Protein Kinase Type 1B, CaM-KI beta, CaMKI-beta, Pregnancy Up-regulated Non-ubiquitously-expressed CaM Kinase, PNCK) |
|
|
Description: Calcium/calmodulin-dependent protein kinase (PNCK) belongs to a proposed calcium-triggered signaling cascade. In vitro it phosphorylates CREB1 and SYN1/synapsin I, and phosphorylates and activates CAMK1 Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:1,000 Western Blot: 1:50-1:100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
|
|||||||||||
|
||||||||||||
|
||||||||||||
|
||||||||||||