Technical Data
C1035-25B9A
CAMK2A, phosphorylated (Thr286) (Calcium/Calmodulin-dependent Protein Kinase Type II Subunit alpha, CaM Kinase II Subunit alpha, CaMK-II Subunit alpha, CAMKA, KIAA0968)
Description:
CaMKII is a member of calcium/calmodulin-activated kinase, functioning in neural synaptic stimulation and T-cell receptor signaling. CaMKII has catalytic and regulatory domains. The binding of Ca++/calmodulin to its regulatory domain releases its autoinhibitory effect and activates the kinase. The activated CaMKII further autophosphorylates itself at Thr286 to render the kinase constitutively active.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1-2ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanRabbit
Concentration:
~0.5mg/ml
Immunogen:
Synthetic phosphopeptide corresponding to Thr286 from human CaMKII.
Purity:
Purified by affinity chromatography.
Form
Supplied as a liquid in PBS, 1% BSA, 0.02% thimerosal, 50% glycerol.
Specificity:
Recognizes human CaMKII when phosphorylated at Thr286. Species Crossreactivity: mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.