		Technical Data
C1035-25B9A	CAMK2A, Phosphorylated (Thr286) (Calcium/Calmodulin-dependent Protein Kinase Type II Subunit alpha, CaM Kinase II Subunit alpha, CaMK-II Subunit alpha, CAMKA, KIAA0968)

Description:
CaMKII is a member of calcium/calmodulin-activated kinase, functioning in neural synaptic stimulation and T-cell receptor signaling. CaMKII has catalytic and regulatory domains. The binding of Ca++/calmodulin to its regulatory domain releases its autoinhibitory effect and activates the kinase. The activated CaMKII further autophosphorylates itself at Thr286 to render the kinase constitutively active.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1-2ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Rabbit

Concentration:

~0.5mg/ml

Immunogen:

Synthetic phosphopeptide corresponding to Thr286 from human CaMKII.

Purity:

Purified by affinity chromatography.

Form

Supplied as a liquid in PBS, 1% BSA, 0.02% thimerosal, 50% glycerol.

Specificity:

Recognizes human CaMKII when phosphorylated at Thr286. Species Crossreactivity: mouse and rat.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.