Technical Data
C1035-26B
Calnexin
Description:
Calnexin (CNX), an abundant ~90kD molecular chaperone, is an unglycosylated resident ER transmembrane protein. In mammalian cells, calnexin, together with calreticulin (CRT), plays a key role in glycoprotein folding and its control within the ER, by interacting with folding intermediates via their monoglucosylated glycans (4). Calnexin associates with newly synthesized monomeric glycoproteins and only recognizes glycoproteins when they are incompletely folded. Calnexin associates with numerous oligomeric protein complexes within the ER, including beta1 and alpha6 integrins (5), major histocompatibility class I (6, 7) and class II molecules (11), the antigen receptors expressed on T and B lymphocytes (7), human thyroperoxidase (hTPO) (12), and acetylcholine receptor (13). Recent data also suggest that calnexin might be responsible for the prolonged retention of pro-alpha6 integrin within ER compartment (14). Furthermore, calnexin has been demonstrated to function as a bonafide molecular chaperone capable of interacting with polypeptide segments of folding glycoproteins (15).

Applications:
Suitable for use in Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry and Electron microscopy. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:1000 (Colorimetric)
Immunohistochemistry: Paraffin sections
Optimal dilutions to be determined by the researcher.

Positive Control:
Heat Shocked HeLa Cell Lysate

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ul-20CBlue IceCanineRabbit
Concentration:
~1mg/ml
Immunogen:
Synthetic peptide corresponding to the C-terminus of canine calnexin. The sequence is completely conserved in human, mouse and rat.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide, 50% glycerol.
Specificity:
Recognizes canine Calnexin. MW:~90kD. Species Crossreactivity: human, monkey, mouse, bovine, chicken, rat, guinea pig, hamster, porcine, quail, rabbit, sheep, Drosophila and Xenopus.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Ou, W.-J., et al., Nature 364: 771-776 (1993). 2. Wada, I., et al., J. Biol. Chem. 266: 19,599-19,610 (1991). 3. Hammond, C. and Helenius, J. Cell. Biol. 126: 41-52 (1994). 4. Elagoz, A., et al., J. Cell Sci. 112: 4449-4460 (1999). 5. Bergeron, J.J., et al., Trends. Biochem. Sci. 19: 124-128 (1994). 6. Bossuyt, X. and Blanckaert, N., Biochem. J. 302: 261-269 (1994). 7. Brunke, M., et al., J. Biol. Chem. 271: 23,487-23,494 (1996). 8. Nelson, D.S., et al., J. Cell Biol. 143: 319-331 (1998). 9. Lu, R. and Misra, V., J. Vir. 74: 934-943 (2000). 10. Kearse, K.P., J. Biol. Chem. 273: 14,152-14,157 (1998). 11. Bu, G. and Rennke, S., J. Biol. Chem. 271: 22,218-22,224 (1996). 12. Fayadat, L., et al., Endocrinology 141(3): 959-966 (2000). 13. Keller, S.H., et al., J. Biol. Chem. 271: 22,871-22,877 (1996). 14. Rigot, V., et al., Eur. J. Biochem. 261(3): 659-666 (1999). 15. Ihara, Y., et al., Mol. Cell 4(3): 331-341 (1999).16. Hobman, T.C., et al., Molec. Biol. of the Cell 9: 1265-1278 (1998). 17. Broquet, A.H., et al., J. Biol. Chem. 278(24): 21,601-21,606 (2003).