Technical Data
Calnexin is calcium-binding protein that interacts with newly synthesized glycoproteins in the endoplasmic reticulum. It may be involved in assisting protein assembly or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the ER quality control apparatus via the retention of incorrectly folded proteins.

Suitable for use in ELISA, Western Blot and Flow Cytometry. Other applications not tested.

Recommended Dilutions:
ELISA: 0.05-0.2ug/ml
Western Blot: 0.5-1ug/ml
Flow Cytometry: 1-3ug to label 1x10e6 cells
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabIgGAffinity Purified
40ug-20CBlue IceHumanRabbit
~0.5mg/ml (after reconstitution)
Synthetic peptide from carboxyl terminal of human calnexin protein, conjugated to KLH (Swiss Prot: P27824). Species Sequence Homology: rat, bovine, canine and Sumatran orangutan
Purified by immunoaffinity chromatography.
Supplied as a lyophilized powder from PBS, pH 7.4, 0.02% sodium azide. Reconstitute with 80ul sterile ddH2O.
Recognizes endogenous levels of human calnexin protein. Species Crossreactivity: mouse
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.