Technical Data
C1035-26J6
Calnexin (CANX, CNX, FLJ26570, Histocompatibility Complex Class I Antigen Binding Protein p88, IP90, P90)
Description:
Calnexin, an abundant ~90kD integral protein of the endoplasmic reticulum, is also referred to as IP90, p88 and p90. It consists of a large 50kD N-terminal calcium-binding luminal domain, a single transmembrane helix and a short acidic cytoplasmic tail. Unlike its ER counterparts which have a KDEL sequence on their C-terminus to ensure ER retention, calnexin has positively charged cytosolic residues that do the same thing. Most ER proteins act as molecular chaperones and participate in the proper folding of polypeptides and their assembly into mulitsubunit proteins. Calnexin together with calreticulin, plays a key role in glycoprotein folding and its control within the ER, by interacting with folding intermediates via their monoglycosylated glycans. Calnexin has also been shown to associate with the major histocompatability complex class I heavy chains, partial complexes of the T cell receptor and B cell membrane immunoglobulin.

Applications:
Suitable for use in Immunofluorescence, ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunofluorescence: 1:200-1:1000
ELISA: 1:10,000
Western Blot: 1:500-1:2000
Immunohistochemistry (Formalin fixed paraffin embedded): 1:200-1:400
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2b3H4A7Ascites
SizeStorageShippingSourceHost
50ul-20CBlue IceHumanMouse
Concentration:
Not determined
Immunogen:
Synthetic peptide CEAAEERPWLWVVYILTVAL corresponding to human Calnexin conjugated to KLH.
Purity:
Ascites
Form
Supplied as a liquid, 0.03% sodium azide.
Specificity:
Recognizes human CANX.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.