Technical Data
C1035-26N
Calnexin (CANX, CNX, FLJ26570, Histocompatibility Complex Class I Antigen Binding Protein p88, IP90, P90)
Description:
Calnexin is a calcium-binding protein that interacts with newly synthesized glycoproteins in the endoplasmic reticulum. As a chaperone protein, it assists in protein assembly by ensuring that only the properly folded protein is progresses along the secretory pathway. It seems to play a major role in the quality control apparatus of the ER by retaining incorrectly folded proteins.

Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:32,000
Western Blot: 0.3-1ug/ml, mouse heart, human brain (cerebellum), NIH3T3 and HeLa lysates on ~100kD bands
Immunohistochemistry (Paraffin): 3-6ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanGoat
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to C-SKTPELNLDQFHDKT, from Calnxin, at the internal region (near N-terminal) of the protein (NP_001737.1).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Specificity:
Recognizes Calnexin. Species Crossreactivity: Human and mouse. Species sequence homology: Bovine, canine, porcine and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Rosser MF, Grove DE, Chen L, Cyr DM, Assembly and misassembly of cystic fibrosis transmembrane conductance regulator: folding defects caused by deletion of F508 occur before and after the calnexin-dependent association of membrane spanning domain (MSD) 1 and MSD2. Molecular biology of the cell 2008 Nov 19(11):4570-9.