		Technical Data
C1035-27A	Calpain I, Large Subunit (Mu Calpain, Protein Kinase C Activating Factor)

Description:
Calpains are cytosolic, calcium-dependent, cysteine proteases that are ubiquitously expressed in mammalian and avian tissues. Type I Calpain requires micromolar amounts of calcium, while type II calpain requires millimolar amounts of calcium.

Applications:
Suitable for use in Western Blotting. Other applications not tested.

Recommended Dilution:
Western Blotting: 0.5-1mg/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Pab	IgG		Serum

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Rabbit

Concentration:

0.2mg/ml

Immunogen:

Human Calpain I (large subunit)

Purity:

Diluted serum

Form

Supplied as a liquid in PBS, 50% glycerol, 1% BSA, 0.02% sodium azide.

Specificity:

Recognizes the large subunit (80kD) of human calpain I. It does not recognize the smaller subunit of human calpain I.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.