Technical Data
C1035-27D
Calpain II, Large Subunit (uCalpain)
Description:
Applications:
Suitable for use in Western Blot, ELISA, Immunohistochemistry and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:100
ELISA: 1:6,000 (against the immunogen) Immunohistochemistry: 1:100 on 4% paraformaldehyde fixed tissue.
Immunocytochemistry: Tested positive on the following human cells: A431 epidermal carcinoma, MDB-MB-134-VI ductal breast carcinoma, C-33A cervical carcinoma, TE2 esophageal carcinoma, HET-1A esophageal carcinoma and normal human monocytes.
Optimal dilution to be determined by researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ul4C (-20C Glycerol)Blue IceHumanRabbit
Concentration:
Not determined
Immunogen:
Human placental m-calpain.
Purity:
Purified by affinity chromatography.
Form
Supplied as a liquid. Contains no preservatives.
Specificity:
Recognizes human m-calpain (Calpain II) large subunit.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Experimental Cell Res. (1992) 203:5-16.