Technical Data
Calpain Small Subunit (Mu Calpain)
Calpains are cytosolic, calcium-dependent, cysteine proteases that are ubiquitously expressed in mammalian and avian tissues. Type I Calpain requires micromolar amounts of calcium, while type II calpain requires millimolar amounts of calcium.

Suitable for use in ELISA, Western Blotting, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Western Blot: 1:500-1000. Recognizes the 30kD small subunits of either calpain isozyme.
ELISA: 1:50,000
Immunohistochemistry: 1:100 on 4% paraformaldehyde or 1% paraformaldehyde/4% glutaraldehyde fixed tissue.
Immunocytochemistry: 1:100
Immunoprecipitation: (small unit, in the presence of calcium large & small complex).
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

100ul-20°CBlue IceBovineMouse
Not determined
Bovine myocardial mu-calpain
Supplied as a liquid. No preservative added.
Reacts with the small subunit of human erthrocyte u-calpain or bovine myocardial m-calpain. Does not react with calpain large subunits
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Zhang, Wenli and Ronald Mellgren (1996) “Calpain subunits remain associated during catalysis.” Biochem. Biophysica. Res. Comm. 227:890–896. Zhang, Wenli (1996) “The major calpain isozymes are long-lived proteins.” J. Biol. Chem. 271:18825–18830. Review: Biochim. Biophys. Acta. (1988) 954:154–160. Intercellular Calcium-Dependent Proteolysis. Ed. R.L. Mellgren, T. Murachi. The Regulation of Calpains by Interaction with Calpastatins. CRC Press, Boca Raton, 1990, p. 115–127.