Technical Data
Calpain (28kD Subunit)
The calpain (calcium-dependent protease or calcium-activated neutral protease) system consists of two ubiquitous forms of calpain (mu-calpain and m-calpain), a tissue specific calpain (n-calpain), and a calpain inhibitory protein (calpastatin). The calpain system has been detected in every vertebrate tissue examined, and has been suggested to play a regulatory role in cellular protein metabolism. This regulatory role may have important implications in platelet aggregation and pathologies associated with altered calcium homeostasis and protein metabolism such as ischemic cell injury and degenerative diseases. Inhibitors of calpain have been shown to block dexamethasone and low-level irradiation induced apoptosis in thymocytes suggesting that calpain has a regulatory or mechanistic role in apoptotic cell death.Mu- and m-calpains are heterodimers consisting of 28kD and 80kD subunits. The 28kD subunit is identical in the two isoforms, but the 80kD subunits differ with ~50% sequence similarity. 28kD / 80kD complexes are thought to be inactive proenzymes which, upon binding of calcium, undergo conformational changes that promotes cleavage of the 28kD subunit and results in enzyme activation.

Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
100ul-20CBlue IceBovineMouse
Not determined
Purified bovine skeletal muscle 28 kDa Calpain subunit.
Supplied as a liquid in PBS, pH 7.2, 0.05% sodium azide
Detects Calpain 28kD subunit from bovine platelet, skeletal and heart muscle and human tissues. This antibody does not crossreact with the human 80kD m-Calpain subunit. Species Crossreactivity: Crossreacts with Human and bovine. Not yet tested in other species.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
This product has been used in:Boehm ML et al. Changes in the calpains and calpastatin during postmortem storage of bovine muscle. J Anim Sci 76:2415-34 (1998).