Technical Data
C1035-27Q
Calpain 1 (Calcium-activated neutral protease, Calcium-activated neutral proteinase 1, Calpain 1 large subunit, CANP, CANP I, CANP1, Capa-1, Capa1, Capn1,mu-Calpain, muCANP, muCL, u-Calpain)
Description:
The calpain (calcium-dependent protease or calcium-activated neutral protease) system consists of two ubiquitous forms of calpain (mu-calpain and m-calpain), a tissue specific calpain (n-calpain), and a calpain inhibitory protein (calpastatin). The calpain system has been detected in every vertebrate tissue examined, and has been suggested to play a regulatory role in cellular protein metabolism. This regulatory role may have important implications in platelet aggregation and pathologies associated with altered calcium homeostasis and protein metabolism such as ischemic cell injury and degenerative diseases. Inhibitors of calpain have been shown to block dexamethasone and low level irradiation induced apoptosis in thymocytes suggesting that calpain has a regulatory or mechanistic role in apoptotic cell death.Mu and M Calpains are heterodimers consisting of 28kD and 80kD subunits. The 28kD subunit is identical in the two isoforms, but the 80kD subunits differ with ~50% sequence similarity. 28kD / 80kD complexes are thought to be inactive proenzymes which, upon binding of calcium, undergo conformational changes that promotes cleavage of the 28kD subunit and results in enzyme activation.

Applications:
Suitable for use in ELISA, Western Blot, Flow Cytometry, Immunocytochemistry and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (frozen): 1:50
Western Blot: 1:500 detects a band at ~80kD representing M Calpain in bovine platelets
Flow Cytometry: 1:50
Immunocytochemistry: staining of M Calpain in LLC-PK1 cells results in diffuse cytoplasmic pattern
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG18J489Ascites
SizeStorageShippingSourceHost
100ul-20CBlue IceBovineMouse
Concentration:
Not determined
Immunogen:
Purified bovine skeletal muscle 80kD subunit of M Calpain. Epitope: aa502-699 (domain III / IV) of human M Calpain. Cellular Localization: Cell membrane; translocates to the plasma membrane upon Ca2+ binding
Purity:
Ascites
Form
Supplied as a liquid, 0.05% sodium azide.
Specificity:
Recognizes the 80kD subunit of M Calpain in bovine platelets, heart and skeletal muscle. Species crossreactivity: human platelets and erythrocytes, rat myoblasts, kidney, liver and spleen and cultured porcine cells. Does not crossreact with Mu Calpain, N Calpain, calmodulin or calpastatin.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
This product has been used in:Boehm ML et al. Changes in the calpains and calpastatin during postmortem storage of bovine muscle. J Anim Sci 76:2415-34 (1998). Proc. 2:547-549, (1995). Cong J et al. Effect of monoclonal antibodies specific for the 28-kDa subunit on catalytic properties of the calpains. J Biol Chem 268:25740-7 (1993). Lane RD et al. A comparison of the intracellular distribution of mu-calpain, m-calpain, and calpastatin in proliferating human A431 cells. Exp Cell Res 203:5-16 (1992).